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Rapid expression of transgenes driven by seed-specific constructs in leaf tissue: DHA production
James R Petrie, Pushkar Shrestha, Qing Liu, Maged P Mansour, Craig C Wood, Xue-Rong Zhou, Peter D Nichols, Allan G Green, Surinder P Singh
Plant Methods 2010, 6:8 (11 March 2010)
[Abstract] [Provisional PDF]
Methodology
Dealing with the problem of non-specific in situ mRNA hybridization signals associated with plant tissues undergoing programmed cell death
Jaana Vuosku, Suvi Sutela, Mira Sääskilahti, Johanna Kestilä, Anne Jokela, Tytti Sarjala, Hely Häggman
Plant Methods 2010, 6:7 (5 February 2010)
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Methodology
Proposal for field sampling of plants and processing in the lab for environmental metabolic fingerprinting
Tanja S Maier, Jürgen Kuhn, Caroline Müller
Plant Methods 2010, 6:6 (29 January 2010)
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Methodology
A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine
Edit Kotogány, Dénes Dudits, Gábor V Horváth, Ferhan Ayaydin
Plant Methods 2010, 6:5 (28 January 2010)
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Methodology
Documentation system for plant transformation service and research
Karin I Köhl, Jürgen Gremmels
Plant Methods 2010, 6:4 (27 January 2010)
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Methodology
NEATTILL: A simplified procedure for nucleic acid extraction from arrayed tissue for TILLING and other high-throughput reverse genetic applications
Yellamaraju Sreelakshmi, Soni Gupta, Reddaiah Bodanapu, Vineeta Singh Chauhan, Mickey Hanjabam, Sherinmol Thomas, Vijee Mohan, Sulabha Sharma, Rajeswari Srinivasan, Rameshwar Sharma
Plant Methods 2010, 6:3 (26 January 2010)
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Methodology
High frequency, cell type-specific visualization of fluorescent-tagged genomic sites in interphase and mitotic cells of living Arabidopsis plants
Antonius JM Matzke, Koichi Watanabe, Johannes van der Winden, Ulf Naumann, Marjori Matzke
Plant Methods 2010, 6:2 (19 January 2010)
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Methodology
Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology
Jian-Feng Li, Li Li, Jen Sheen
Plant Methods 2010, 6:1 (14 January 2010)
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Methodology
Impact of ubiquitous inhibitors on the GUS gene reporter system: evidence from the model plants Arabidopsis, tobacco and rice and correction methods for quantitative assays of transgenic and endogenous GUS
Simone Fior, Paolo D Gerola
Plant Methods 2009, 5:19 (30 December 2009)
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Software
Computer aided data acquisition tool for high-throughput phenotyping of plant populations
Raju Naik Vankadavath, Appibhai Jakir Hussain, Reddaiah Bodanapu, Eros Kharshiing, Pinjari Osman Basha, Soni Gupta, Yellamaraju Sreelakshmi, Rameshwar Sharma
Plant Methods 2009, 5:18 (10 December 2009)
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Methodology
A rapid and sensitive method for the simultaneous analysis of aliphatic and polar molecules containing free carboxyl groups in plant extracts by LC-MS/MS
Mario Kallenbach, Ian T Baldwin, Gustavo Bonaventure
Plant Methods 2009, 5:17 (25 November 2009)
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Methodology
Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method
Fu-Hui Wu, Shu-Chen Shen, Lan-Ying Lee, Shu-Hong Lee, Ming-Tsar Chan, Choun-Sea Lin
Plant Methods 2009, 5:16 (24 November 2009)
[Abstract] [Full Text] [PDF] [PubMed] [Related articles] [F1000 Biology]
Methodology
An inbred line of the diploid strawberry Fragaria vesca f. semperflorens for genomic and molecular genetic studies in the Rosaceae
Janet P Slovin, Kyle Schmitt, Kevin M Folta
Plant Methods 2009, 5:15 (31 October 2009)
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Methodology
A simple, flexible and efficient PCR-fusion/Gateway cloning procedure for gene fusion, site-directed mutagenesis, short sequence insertion and domain deletions and swaps
Ivan I Atanassov, Ilian I Atanassov, J Peter Etchells, Simon R Turner
Plant Methods 2009, 5:14 (28 October 2009)
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Methodology
Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations
Antoine LF Gady, Freddy WK Hermans, Marion HBJ Van de Wal, Eibertus N van Loo, Richard GF Visser, Christian WB Bachem
Plant Methods 2009, 5:13 (7 October 2009)
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Methodology
Micro-scaled high-throughput digestion of plant tissue samples for multi-elemental analysis
Thomas H Hansen, Kristian H Laursen, Daniel P Persson, Pai Pedas, Søren Husted, Jan K Schjoerring
Plant Methods 2009, 5:12 (26 September 2009)
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Methodology
3D fluorescent in situ hybridization using Arabidopsis leaf cryosections and isolated nuclei
Leïla Tirichine, Philippe Andrey, Eric Biot, Yves Maurin, Valérie Gaudin
Plant Methods 2009, 5:11 (3 August 2009)
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Methodology
High-throughput retrotransposon-based fluorescent markers: improved information content and allele discrimination
Maggie Knox, Carol Moreau, James Lipscombe, David Baker, Noel Ellis
Plant Methods 2009, 5:10 (28 July 2009)
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Methodology
Development of a gene silencing DNA vector derived from a broad host range geminivirus
Edward M Golenberg, D Noah Sather, Leandria C Hancock, Kenneth J Buckley, Natalie M Villafranco, David M Bisaro
Plant Methods 2009, 5:9 (2 July 2009)
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Software
The DAWGPAWS pipeline for the annotation of genes and transposable elements in plant genomes
James C Estill, Jeffrey L Bennetzen
Plant Methods 2009, 5:8 (19 June 2009)
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